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KMID : 0380219930260050465
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 5 p.465 ~ p.472
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Reconstitution of Sarcoplasmic Reticula Ca2+-ATPases from Cardiac and Skeletal Muscle
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Hae Won Kim
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Abstract
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Reconstitution of cardiac and skeletal sarcoplasmic reticula (SR) Ca2+-ATPases into liposomes requires different conditioos for optimum enzymatic activity. In the presence of phosphatidylcholine (PC), maximum activity was obtasined at a
cholate/Ca2+-ATPase/PC ratio (w/w) of 2/1/80 for cardiac SR Ca2+-ATPase, and 4.5/1/40 for skeletal SR Ca2+-ATPase. Titution buffers, while this was essential for skeletal Ca2+-ATPase. The maximum rates of Ca2+. Uptake by
reconstitute vesicles were 700nmol/mg/min for cardiac Ca2+-ATPase, and 960 nmol/mg min for skeletal Ca2+-ATPase. These findings suggest that Ca2+-ATPases from cardiac and skeletal SR required different conditions for
rreconstitution,
even
though they have similar amino acid sequences (80% homology) and phospholipid compositions.
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KEYWORD
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